HomehealthScientists Uncover Secrets of...

Scientists Uncover Secrets of Plant Regeneration

Research Summary

The researchers identified a key negative regulator of stem regeneration, WOX13, which promotes the fate of non-meristematic cells through a transcriptional repressor, thereby affecting regeneration efficiency. This finding provides new insights into cell fate specification pathways and suggests that knockdown of WOX13 can improve stem regeneration efficiency, which could be a valuable tool in agriculture and horticulture.

 

 

 

Japanese researchers have discovered that the WOX13 gene negatively controls the fate of plant cell regeneration and affects the efficiency of plant stem regeneration.

 

Plants possess the unique ability to regenerate completely from a somatic cell, that is, a normal cell that normally does not participate in reproduction. This process involves the de novo (or new) formation of a shoot apical meristem (SAM), which generates lateral organs, which are key to plant reconstruction.

 

At the cellular scale, SAM formation is tightly controlled by positive or negative regulators (genes/protein molecules) that induce or limit stem regeneration, respectively. But which molecules are involved? Are there other regulatory layers that have yet to be discovered? To find answers to the above questions, a research team led by the Nara Institute of Science and Technology (NAIST) in Japan investigated this process in Arabidopsis thaliana, a plant commonly used in genetic research.

 

Their study, published in Science Advances, discovered and characterized a key negative regulator of shoot regeneration. They show how the USCHEL-associated HOMEOBOX 13 (WOX13) gene and its protein affect regeneration efficiency by acting as transcriptional (RNA level) repressors that promote the non-meristematic (non-dividing) function of callus cells.

 

“The search for strategies to improve the efficiency of plant stem regeneration has been ongoing for a long time. However, progress has been hampered because the relevant regulatory mechanisms are unclear. Our study fills this gap by defining a new cell fate specification pathway blank,” explains Momoko Ikeuchi, lead researcher on the study. Previous research by her team had identified a role for WOX13 in tissue repair and organ adhesion after transplantation. Therefore, they first tested this gene’s potential role in controlling stem regeneration in WOX13 Arabidopsis mutants (plants with dysfunctional WOX13) using a two-step tissue culture system.

 

Phenotypic and imaging analyzes showed that shoot regeneration was accelerated (3 days faster) in plants lacking WOX13, whereas it was slower in plants induced to express WOX13. Furthermore, in normal plants, WOX13 was locally expressed at reduced levels in SAMs. The above results indicated that WOX13 has a negative regulatory effect on stem regeneration.

 

To validate their findings, the researchers compared WOX13 mutants to wild-type (normal) plants using RNA sequencing at multiple time points. Under callus-inducing conditions, deletion of WOX13 did not significantly change Arabidopsis gene expression. However, shoot-inducing conditions significantly enhanced the changes induced by the WOX13 mutation, resulting in the upregulation of shoot meristem regulatory genes.

 

Interestingly, in mutant plants, these genes were repressed within 24 hours of WOX13 overexpression. Collectively, they found that WOX13 represses a subset of stem meristem regulators while directly activating cell wall modifying genes involved in cell expansion and cell differentiation. Subsequent Quartz-Seq2-based single-cell RNA sequencing (scRNA-seq) confirmed that WOX13 plays a key role in pluripotent callus cell fate.

 

This study highlights that, unlike other known negative regulators of shoot regeneration, which only prevent callus transition to SAM, WOX13 inhibits SAM specification by promoting the acquisition of alternative fates. It achieves this suppression through a regulatory circuit that reciprocally inhibits the regulatory factor WUS, which promotes the fate of non-meristematic cells by inducing cell wall modification factors through transcriptional repression of WUS and other SAM regulators.

 

Thus, WOX13 is a master regulator of regeneration efficiency. “Our results show that knocking out WOX13 can promote the acquisition of shoot fate and improve the efficiency of shoot regulation. This means that WOX13 knockout can be used as a tool in agriculture and horticulture to promote tissue culture-mediated regeneration of new crop shoots,” concluded Ikeuchi.

- A word from our sponsors -

spot_img

Most Popular

LEAVE A REPLY

Please enter your comment!
Please enter your name here

More from Author

How to Improve Your Golf Swing: Tips and Techniques

Golf Swing Drills Improving your golf swing is a constant endeavor for...

Saving Money on Car Insurance

Discover a roadmap to savings on your car insurance with our latest blog. Learn strategic tips on trimming down costs without compromising coverage. From shopping around for the best rates to bundling policies, maintaining a clean driving record, and exploring various discounts, this guide empowers you to make informed decisions to keep your car insurance expenses within budget.

CRM Design and Development Services & Solutions by NOS Digital in Dubai, UAE

In the dynamic landscape of business, customer relationship management (CRM) plays...

Unveiling the Best Wedding Planners in Jaipur

Introduction: A wedding is a cherished milestone, a celebration of love,...

- A word from our sponsors -

spot_img

Read Now

How to Improve Your Golf Swing: Tips and Techniques

Golf Swing Drills Improving your golf swing is a constant endeavor for any golfer, regardless of their skill level. A solid golf swing is the foundation of a successful game, and mastering it requires practice and dedication. In this article, we will explore various golf swing drills that...

Saving Money on Car Insurance

Discover a roadmap to savings on your car insurance with our latest blog. Learn strategic tips on trimming down costs without compromising coverage. From shopping around for the best rates to bundling policies, maintaining a clean driving record, and exploring various discounts, this guide empowers you to make informed decisions to keep your car insurance expenses within budget.

CRM Design and Development Services & Solutions by NOS Digital in Dubai, UAE

In the dynamic landscape of business, customer relationship management (CRM) plays a pivotal role. NOS Digital, based in Dubai, UAE, stands out as a leading provider of CRM Design and Development Services & Solutions. This article delves into the comprehensive offerings by NOS Digital, showcasing the expertise,...

Unveiling the Best Wedding Planners in Jaipur

Introduction: A wedding is a cherished milestone, a celebration of love, unity, and the promise of a beautiful future. In the heart of Rajasthan, the Pink City, Jaipur, stands as a regal backdrop for couples seeking a magical setting for their special day. To turn dreams into...

The Timeless Allure of Stainless Steel Spigots for Glass Fencing

This article will tell you about all the benefits of Stainless Steel Spigots.

Car Rental in Dubai: Your Ultimate Guide to Cheap Rent a Car in the City of Luxury

Introduction Dubai, a city known for its opulence, futuristic skyline, and vibrant culture, is a destination that beckons travellers from around the world. As you plan your visit to this dazzling city, one crucial aspect to consider is transportation. While public transportation is available, nothing beats the convenience...

The Best Ideas for Birthday Invitation Card Designs

Elevate your celebrations with creative birthday invitation party card ideas. From personalized touches to interactive elements, discover the best designs now!

Unveiling the True Cost: How Much Do Custom T-Shirts Cost?

In this article, we will explore the intricacies of custom t-shirt pricing, shedding light on the various elements that contribute to the final cost.

PureKonect with Gershom Sikalaa: Growing Social Networking

Introduction: Under the strong supervision of Gershom Sikalaa, PureKonect represents introducers for those smart users of social networking. The article describes PureKonect, how the method affects its primary features, and how Sikalaa's future plans will affect the process by which users register for online platforms. Sikalaa Who is...

Unraveling the Web of (SEO) Search Engine Optimization

Title: Understanding the Essence of SEO: Unraveling the Web of Search Engine Optimization Introduction: In the vast and dynamic landscape of the internet, where information flows ceaselessly, the prominence of Search Engine Optimization (SEO) has become more vital than ever. SEO is the driving force behind ensuring that a...

Experience Seamless Connectivity with RDP Singapore

Introduction In the fast-paced digital landscape, Remote Desktop Protocol (RDP) in Singapore has emerged as a cornerstone for seamless connectivity. This article delves into the intricacies of RDP, offering insights and expertise to empower users with a robust understanding of this technology. The Evolution of RDP Remote Desktop Protocol has...

What Are the Advantages of a Hair Transplant in Dubai?

Introduction Hair transplant, a cosmetic procedure that involves moving hair follicles from one part of the body (the donor site) to a bald or balding part (the recipient site), has witnessed a surge in demand globally. In Dubai, this trend is not merely catching on; it's thriving. Let's...